elisa kit  (Cusabio)

Journal: Molecular Neurobiology

Article Title: The Receptor for Advanced Glycation End-products in the Mouse Anterior Cingulate Cortex is Involved in Neuron‒Astrocyte Coupling in Chronic Inflammatory Pain and Anxiety Comorbidity

doi: 10.1007/s12035-025-04713-y

Figure Lengend Snippet: RAGE expression in the ACC is upregulated at 9d after CFA treatment. a , Progression of the thermal withdrawal latency (TWL) and mechanical withdrawal threshold (MWT) following intraplantar CFA or saline injection. CFA-R: the right hindpaws of CFA-treated mice contralateral to the CFA injection. BL: baseline. b , Expression level of RAGE in the ACC at different stages after intraplantar CFA or saline injection, as measured by ELISA. c , Immunofluorescence of RAGE in ACC neurons at different stages after intraplantar CFA or saline injection (scale bar = 50 μm). d , Count numbers of RAGE-positive neurons. e , Schematic diagram showing where the immunofluorescence images were taken. ** p < 0.01. ns: not significant

Article Snippet: The subsequent steps followed the instructions of the mouse RAGE ELISA kit (EK2103, Hangzhou Multiscience Co., Ltd., CN) and mouse S100B ELISA kit (MM-45105M2, Jiangsu Meimian Industrial Co., Ltd., CN).

Techniques: Expressing, Saline, Injection, Enzyme-linked Immunosorbent Assay, Immunofluorescence

Journal: Molecular Neurobiology

Article Title: The Receptor for Advanced Glycation End-products in the Mouse Anterior Cingulate Cortex is Involved in Neuron‒Astrocyte Coupling in Chronic Inflammatory Pain and Anxiety Comorbidity

doi: 10.1007/s12035-025-04713-y

Figure Lengend Snippet: Intra-ACC injection of FPS-ZM1 decreases RAGE expression and alleviates hypersensitivity and anxiety in CIP mice. a , Schematic diagram showing where and when FPS-ZM1 or vehicle was injected. b , Expression level of RAGE in the ACC, including the saline control group and CFA groups, after FPS-ZM1 or vehicle injection, as measured by ELISA. c, Immunofluorescence of RAGE in ACC neurons from the saline control group and the CFA groups after FPS-ZM1 or vehicle injection (scale bar = 50 μm). d , Count numbers of RAGE-positive neurons. e , Thermal withdrawal latency (TWL) and mechanical withdrawal threshold (MWT), including those of the saline control group and the CFA groups after FPS-ZM1 or vehicle injection. f , Heatmap of the elevated plus maze, including the saline control group and the CFA groups, after FPS-ZM1 or vehicle injection (the cross arm of the map is the closed arm, and the longitudinal arm is the open arm). g , Calculation of the percentage of time spent in the open arm (RTOA) and the percentage of distance traveled in the open arm (DOA) by the mice in the elevated plus maze. h , Heatmap of the open field test results, including those of the saline control group and the CFA groups, after FPS-ZM1 or vehicle injection. i , Calculation of the percentage of mouse retention time in the central area (RTCA) and the percentage of mouse distance traveled in the central area (DCA) in the open field test. * p < 0.05, ** p < 0.01

Article Snippet: The subsequent steps followed the instructions of the mouse RAGE ELISA kit (EK2103, Hangzhou Multiscience Co., Ltd., CN) and mouse S100B ELISA kit (MM-45105M2, Jiangsu Meimian Industrial Co., Ltd., CN).

Techniques: Injection, Expressing, Saline, Control, Enzyme-linked Immunosorbent Assay, Immunofluorescence

Journal: Molecular Neurobiology

Article Title: The Receptor for Advanced Glycation End-products in the Mouse Anterior Cingulate Cortex is Involved in Neuron‒Astrocyte Coupling in Chronic Inflammatory Pain and Anxiety Comorbidity

doi: 10.1007/s12035-025-04713-y

Figure Lengend Snippet: S100B was upregulated at 9d after CFA treatment, and intra-ACC injection of S100B activated RAGE but did not induce pain-related behavioral changes under normal conditions. a , Immunofluorescence of S100B in ACC astrocytes at different stages after intraplantar CFA or saline injection (scale bar = 50 μm). b , Count numbers of S100B-positive cells. c , Expression level of S100B in the ACC at different stages after intraplantar CFA or saline injection, as measured by ELISA. d , Schematic diagram showing where and when S100B or vehicle was injected into saline-treated mice. e , Immunofluorescence of RAGE in the ACC after intra-ACC injection of S100B or vehicle (scale bar = 50 μm). f , Count numbers of RAGE-positive cells. g , Expression level of RAGE in the ACC after intra-ACC injection of S100B or vehicle, as measured by ELISA. h , Thermal withdrawal latency (TWL) and mechanical withdrawal threshold (MWT) of saline-treated mice after S100B or vehicle injection. i , Heatmap of saline-treated mice in the elevated plus maze after S100B or vehicle injection (the cross arm of the map is the closed arm, and the longitudinal arm is the open arm). j , Calculation of the percentage of time spent in the open arm (RTOA) and the percentage of distance traveled in the open arm (DOA) by the mice in the elevated plus maze. k , Heatmap of saline-treated mice in the open field test after S100B or vehicle injection. l , Calculation of the percentage of mouse retention time in the central area (RTCA) and the percentage of mouse distance traveled in the central area (DCA) in the open field test.** p < 0.01. ns: not significant

Article Snippet: The subsequent steps followed the instructions of the mouse RAGE ELISA kit (EK2103, Hangzhou Multiscience Co., Ltd., CN) and mouse S100B ELISA kit (MM-45105M2, Jiangsu Meimian Industrial Co., Ltd., CN).

Techniques: Injection, Immunofluorescence, Saline, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Molecular Neurobiology

Article Title: The Receptor for Advanced Glycation End-products in the Mouse Anterior Cingulate Cortex is Involved in Neuron‒Astrocyte Coupling in Chronic Inflammatory Pain and Anxiety Comorbidity

doi: 10.1007/s12035-025-04713-y

Figure Lengend Snippet: Intra-ACC injection of L-α-AA reduces RAGE levels and alleviates hypersensitivity and anxiety in CFA-treated mice. a , Schematic diagram showing the experimental protocol. b , Expression level of S100B in the ACC after L-α-AA or vehicle injection, as measured by ELISA. c , Count numbers of S100B-positive neurons. d , Immunofluorescence of S100B in ACC astrocytes after L-α-AA or vehicle injection (scale bar = 50 μm). e , Immunofluorescence of RAGE in ACC neurons after L-α-AA or vehicle injection (scale bar = 50 μm). f , Count numbers of RAGE-positive neurons. g , Expression level of RAGE in the ACC after L-α-AA or vehicle injection, as measured by ELISA. h , Thermal withdrawal latency (TWL) and mechanical withdrawal threshold (MWT) of CFA-treated mice after L-α-AA or vehicle injection. i , Heatmap of CFA-treated mice in the elevated plus maze after L-α-AA or vehicle injection (the cross arm of the map is the closed arm, and the longitudinal arm is the open arm). j , Calculation of the percentage of time spent in the open arm (RTOA) and the percentage of distance traveled in the open arm (DOA) by the mice in the elevated plus maze. k , Heatmap of CFA-treated mice in the open field test after L-α-AA or vehicle injection. l , Calculation of the percentage of mouse retention time in the central area (RTCA) and the percentage of mouse distance traveled in the central area (DCA) in the open field test. * p < 0.05, ** p < 0.01

Article Snippet: The subsequent steps followed the instructions of the mouse RAGE ELISA kit (EK2103, Hangzhou Multiscience Co., Ltd., CN) and mouse S100B ELISA kit (MM-45105M2, Jiangsu Meimian Industrial Co., Ltd., CN).

Techniques: Injection, Expressing, Enzyme-linked Immunosorbent Assay, Immunofluorescence

Journal: iScience

Article Title: Weight-neutral early time-restricted eating improves glycemic variation and time in range without changes in inflammatory markers

doi: 10.1016/j.isci.2024.111501

Figure Lengend Snippet:

Article Snippet: Human RAGE ELISA Kit - Quantikine , R&D Systems , DRG00.

Techniques: Enzyme-linked Immunosorbent Assay, Software